Saturday, April 11, 2009

How can the Beer Lambert Law be used to determine the concentration of a substance in solution?

If you know the molar absorbance coefficient of a particular substance, how can the Beer-Lambert Law be used to determine the concentration of a substance in solution?


Beer Lambert Law: A=E.b.c

You have the the solution...measure its absorbance(A), you know the absorbace coefficient(E), you know the path length of the cell used to measure absorbance(b). Now rearrange the equation to get c...

c = A/(E.b)

done kthnx

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